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S-RMab®CD8α Recombinant Rabbit mAb (SDT-142-52)

T-cell surface glycoprotein CD8 alpha chain,T-cell surface glycoprotein Lyt-2,Lyt-2,Lyt2

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0034
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产品介绍

产品介绍 FAQs 引用文献(6) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    CD8α

  • 分子别名

    T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, Lyt-2, Lyt2

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cell membrane

  • Accession

    P01731

  • 克隆号

    SDT-142-52

  • 抗体类型

    Rabbit mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ?

    FCM

    WB

    IF ?

  • 稀释度

    应用 稀释度
    FCM 1:50
    WB 1:1000
    IF 1:1000
    IHC-P 1:500
背景介绍

  • CD8A encodes the CD8 alpha chain of the αβT cells, proposed as a quantifiable indicator for CD8+ CTL recruitment or activity assessments and a robust biomarker for responses to anti-PD-1/PD-L1 therapy.In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.

  • 免疫印迹

    • WB result of CD8α Rabbit mAb
      Primary antibody: CD8α Rabbit mAb at 1/1000 dilution
      Lane 1: RAW 264.7 whole cell lysate 20 µg
      Lane 2: mouse thymus lysate 20 µg
      Negative control: RAW 264.7 whole cell lysate

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 29 kDa
      Observed MW: 28~37 kDa
      Exposure time: 60s

  • 流式分析

    • Flow cytometric analysis of mouse primary splenocytes labeling CD8α antibody at 1/50 (1 μg) dilution / (right panel) compared with a Rabbit IgG, Isotype Control / (left panel). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
      Cells were surface stained with CD4-Alexa Fluor® 647, then stained with rabbit IgG (Left) / CD8α (Right) separately. CD4 and CD8α are mutually exclusive expressed in mouse spleen. Gated on total viable cells.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded mouse thymus. Anti-CD8α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD8α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse colon. Anti-CD8α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • Negative control: IHC shows negative staining in paraffin-embedded mouse cardiac muscle. Anti-CD8α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫荧光

    • IF shows positive staining in paraffin-embedded mouse thymus. Anti-CD8α antibody was used at 1/1000 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with ICC staining protocol. Nuclei were counterstained with DAPI.

FAQs

斯达特公司的抗体，可以回收利用几次？

我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定

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