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SMAD4 Recombinant Rabbit mAb (SDT-168-67)

Mothers against decapentaplegic homolog 4,MAD homolog 4,Mothers against DPP homolog 4,Deletion target in pancreatic carcinoma 4,hSMAD4

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B0033

规格

25μl100μl1ml

数量

产品介绍

产品介绍 FAQs 评论(0)

产品规格

宿主来源

Rabbit
抗原名称

Smad4
分子别名

Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; hSMAD4
免疫原

Synthetic Peptide
细胞定位

Cytoplasm, Nucleus
Accession

Q13485
克隆号

SDT-168-67
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
预测反应种属
(反应种属缩写表)

Bv, Pg
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ChIP

WB

IP
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
IP 1:50 Hu, Ms, Rt
ChIP 1:20 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
IP	1:50	Hu, Ms, Rt
ChIP	1:20	Hu

背景介绍

SMAD (mothers against decapentaplegic homologs) molecules are the core components in TGF-β signaling pathway. TGF-β binding to its receptor induces phosphorylation and activation of receptor-regulated SMADs (R-SMADs), SMAD2 and SMAD3, which subsequently associate with their partner SMAD4 and translocate from cytoplasm to nucleus. Formation of R-SMAD–SMAD4 complexes is essential in signaling of most TGF-β family members.

免疫印迹
- WB result of Smad4 Rabbit mAb
  Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
  Lane 1: SW480 whole cell lysate 20 µg
  Lane 2: HT-29 whole cell lysate 20 µg
  Lane 3: HeLa whole cell lysate 20 µg
  Lane 4: HepG2 whole cell lysate 20 µg
  Lane 5: Jurkat whole cell lysate 20 µg
  Negative control: SW480 whole cell lysate;
  HT-29 whole cell lysate
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 70 kDa
  Observed MW: 65 kDa
  Exposure time: 180s
- WB result of Smad4 Rabbit mAb
  Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
  Lane 1: NIH/3T3 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 70 kDa
  Observed MW: 65 kDa
  Exposure time: 180s
- WB result of Smad4 Rabbit mAb
  Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
  Lane 1: C6 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 70 kDa
  Observed MW: 65 kDa
  Exposure time: 180s
免疫沉淀
- SMAD4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SMAD4 in 0.4 mg HeLa whole cell lysate.
  Western blot was performed on the immunoprecipitate using SMAD4 Rabbit mAb at 1/1000 dilution.
  Secondary antibody (HRP) for IP was used at 1/1000 dilution.
  Lane 1: HeLa whole cell lysate 20 µg (Input)
  Lane 2: SMAD4 Rabbit mAb IP in HeLa whole cell lysate
  Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
  Predicted MW: 70 kDa
  Observed MW: 70 kDa
ChIP
- Chromatin immunoprecipitation (ChIP) was performed on HaCaT+TGF-β(7ng/mL,1h) cells cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used SMAD4 Recombinant Rabbit mAb (SDT-168-67) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:20 for immunoprecipitation.
  Post - immunoprecipitation, both samples were washed, eluted, and cross - links reversed. Purified DNA was analyzed by qPCR.
  qPCR showed the enrichment of ID1, CDKN1A and SAT-α in SMAD4 Recombinant Rabbit mAb (SDT-168-67)-immunoprecipitated sample.