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产品规格
宿主来源
Rabbit抗原名称
GSTP-1分子别名
Glutathione S-Transferase Pi, GST-pi, GST class-pi, GSTP1-1细胞定位
Cytoplasm, NucleusAccession
P09211克隆号
SDT-R054抗体类型
Rabbit mAb反应种属 ?
Hu, Ms, Rt纯化方式
Protein A浓度
1 mg/ml性状
Liquid缓冲体系
PBS
储存条件
12 months from date of receipt, 4°C as supplied
应用
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 WB 1:500-1:2500 IHC-P 1:2000 ICC 1:500-1:1000
背景介绍
GST-π is abundantly expressed in some mammalian tissues, particularly those associated with malignancies. While the enzyme can catalyze thioether bond formation between some electrophilic chemicals and GSH, novel non-detoxification functions are now ascribed to it.
免疫印迹
- WB result of GST-π Rabbit mAb Primary antibody: GST-π Rabbit mAb at 1/2500 dilution Lane 1: LNCaP whole cell lysate 20 µg Lane 2: K-562 whole cell lysate 20 µg Negative control: LNCaP whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 23 kDa Observed MW: 23 kDa Exposure time: 180s
- WB result of GST-π Rabbit mAb Primary antibody: GST-π Rabbit mAb at 1/2500 dilution Lane 1: LNCaP whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Negative control: LNCaP whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 23 kDa Observed MW: 23 kDa Exposure time: 180s
- WB result of GST-π Rabbit mAb Primary antibody: GST-π Rabbit mAb at 1/500 dilution Lane 1: mouse liver lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 23 kDa Observed MW: 23 kDa Exposure time: 180s
- WB result of GST-π Rabbit mAb Primary antibody: GST-π Rabbit mAb at 1/500 dilution Lane 1: rat kidney lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 23 kDa Observed MW: 23 kDa Exposure time: 180s
免疫组化
- IHC shows positive staining in paraffin-embedded human kidney. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded mouse liver. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
- IHC shows positive staining in paraffin-embedded rat kidney. Anti-GST-π antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in K562 cells. Anti-GST-π antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
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