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宿主来源
Rabbit抗原名称
LIV-1分子别名
Zinc transporter ZIP6, Estrogen-regulated protein LIV-1, Solute carrier family 39 member 6, Zrt- and Irt-like protein 6, ZIP-6免疫原
Synthetic Peptide细胞定位
Membrane, CytoplasmAccession
Q13433克隆号
SDT-206-84抗体类型
Rabbit mAb应用
IHC-P, WB反应种属 ?
Hu预测反应种属
(反应种属缩写表)Or纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
| 应用 | 稀释度 |
|---|---|
| WB | 1:1000 |
| IHC-P | 1:200 |
Zinc transporter LIV-1 (SLC39A6) is estrogen regulated and present in increased amounts in estrogen receptor-positive breast cancer as well as in tumors that spread to the lymph nodes. The LIV-1 subfamily of ZIP zinc transporters consists of nine human sequences that share considerable homology across transmembrane domains. Many of these sequences have been shown to transport zinc and/or other ions across cell membranes. SLC39A6 is a member of the ZIP family of transporters, which control zinc homeostasis by regulating the influx of zinc from extracellular to intracellular spaces. The zinc transport function of SLC39A6 plays an important role in cellular metabolism. Zinc is required for a variety of cellular processes, including immune activity, protein synthesis, nucleic acid metabolism, cell proliferation, tissue repair and cell division, and low zinc levels can lead to metabolic disorder and inhibit cell growth. Overexpression of SLC39A6 has been related to the progression of several types of cancer, including breast , prostate, pancreatic, cervical and liver cancer, verexpression of SLC39A6 promoted the epithelial-mesenchymal transition (EMT).
免疫印迹
WB result of LIV-1/SLC39A6 Rabbit mAb
Primary antibody: LIV-1/SLC39A6 Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 16 µg
Lane 2: MCF7 whole cell lysate (deglycosylated with PNGase F) 16 μgSecondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 85 kDa
Observed MW: 103/85/68 kDa
Exposure time: 180s
免疫组化
IHC shows positive staining in paraffin-embedded human breast cancer.
Anti-LIV-1/SLC39A6 antibody was used at 1/200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer.
Anti-LIV-1/SLC39A6 antibody was used at 1/200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast. Anti-LIV-1/SLC39A6 antibody was used at 1/200 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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