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宿主来源
Rabbit抗原名称
Lamin B2分子别名
LMNB2,LMN2免疫原
Synthetic Peptide细胞定位
Nucleus laminaAccession
Q03252克隆号
SDT-061-47抗体类型
Rabbit mAb反应种属 ?
Hu纯化方式
Protein A浓度
0.25 mg/ml性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.
应用
IHC-P ?
WB
IP
稀释度
应用 稀释度 WB 1:500 IHC-P 1:1000 FCM 1:250 IP 1:25
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin B2 is a protein that in humans is encoded by the LMNB2 gene. It is the second of two type B nuclear lamins, and it is associated with laminopathies.
免疫印迹
WB result of Lamin B2 Rabbit mAb
Primary antibody: Lamin B2 Rabbit mAb at 1/500 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µgSecondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 68 kDa
免疫沉淀
Lamin B2 Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating Lamin B2 in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using Lamin B2 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HepG2 whole cell lysate 10 µg (Input)
Lane 2: Lamin B2 Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 50 kDa
Observed MW: 68 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human stomach.
Anti-Lamin B2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex.
Anti-Lamin B2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer.
Anti-Lamin B2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer.
Anti-Lamin B2 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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