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Rabbit抗原名称
HSP60分子别名
60 kDa chaperonin, Chaperonin 60 (CPN60), Heat shock protein 60 (HSP-60; Hsp60) HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, HSPD1免疫原
Synthetic Peptide细胞定位
IntracellularAccession
P10809克隆号
SDT-076-6抗体类型
Rabbit mAb反应种属 ?
Hu, Ms, Rt预测反应种属
(反应种属缩写表)Hm纯化方式
Protein A浓度
0.5mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied.
应用
ICFCM
IHC-P ?
ICC ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:20000 Hu, Ms, Rt IHC-P 1:2000 Hu, Ms, Rt ICFCM 1:500 Hu ICC 1:1000 Hu, Ms
HSP60 is primarily a mitochondrial protein and is important for the folding of key proteins upon entry into the mitochondria. Studies have shown that a large amount of HSP60 is also present in the cytoplasm of many cells, and the inducers of HSP60 are stress, inflammation and immune response.
免疫印迹
- WB result of Hsp60 Rabbit mAb Primary antibody : Hsp60 Rabbit mAb at 1/1000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: 293T whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 60 kDa Observed MW: 60 kDa Exposure time: 3s
WB result of Hsp60 Rabbit mAb
Primary antibody : Hsp60 Rabbit mAb at 1/1000 dilution
Lane 1: NIH3T3 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µgSecondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Exposure time: 10sWB result of Hsp60 Rabbit mAb
Primary antibody : Hsp60 Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µgSecondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Exposure time: 10sWB result of Hsp60 Rabbit mAb
Primary antibody: Hsp60 Rabbit mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Lane 3: rat heart lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 60 kDa
Exposure time: 60 s
流式分析
Flow cytometric analysis of HeLa cells labelling Hsp 60 antibody at 1/500(0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
免疫组化
IHC shows positive staining in paraffin-embedded human liver cancer.
Anti-Hsp60 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver.
Anti-Hsp60 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon.
Anti-Hsp60 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil.
Anti-Hsp60 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer.
Anti-Hsp60 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver.
Anti-Hsp60 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kindey.
Anti-Hsp60 antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows cytoplasm staining in HeLa cells.
Anti-Hsp60 antibody was used at 1/1000 dilution and incubated overnight at 4°C.
Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution.
The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100.
Nuclei were counterstained with DAPI.
ICC shows cytoplasm staining in NIH/3T3 cells.
Anti-Hsp60 antibody was used at 1/1000 dilution and incubated overnight at 4°C.
Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution.
The cells were fixed with 100% methanol and permeabilized with 0.1% PBS-Triton X-100.
Nuclei were counterstained with DAPI.
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