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β-actin Rabbit mAb (SDT-R015)

ACTB

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0005
规格
数量
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产品介绍 引用文献(13) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    β-actin

  • 分子别名

    ACTB

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Cytoplasm, Cytoskeleton

  • Accession

    P60709

  • 克隆号

    SDT-R015

  • 抗体类型

    Rabbit mAb

  • 应用

    ICFCM ,
    IHC-P ? ,
    ICC ? ,
    WB

  • 反应种属 ?

    Hu, Ms, Rat

  • 纯化方式

    Protein A

  • 浓度

    0.5mg/ml

  • 分子量

    42kDa

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied.

稀释度
应用 稀释度
ICC 1:500
WB 1:1000-1:5000
ICFCM 1:500
IHC-P 1:2000
背景介绍

  • Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.

  • 免疫印迹

    • WB result of β-actin Rabbit mAb

      Primary antibody: β-actin Rabbit mAb at 1/1000 dilution
      Lane 1: Hela whole cell lysate 20 µg
      Lane 2: Jurkat whole cell lysate 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa

    • WB result of β-actin Rabbit mAb

      Primary antibody: β-actin Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 lysate 20 µg
      Lane 2: mouse kidney lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa

    • WB result of β-actin Rabbit mAb

      Primary antibody: β-actin Rabbit mAb at 1/1000 dilution
      Lane 1: rat brain lysate 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa

    • WB result of β-actin Rabbit mAb
      Primary antibody: β-actin Rabbit mAb at 1/5000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Lane 3: C6 whole cell lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 42 kDa
      Observed MW: 42 kDa

  • 流式分析

    • Flow cytometric analysis of HeLa cells labelling β-actin antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded smooth msucle of human skeletal muscle.

      Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon.

      Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer.

      Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human lung cancer.

      Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver.

      Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat cerebral cortex.

      Anti-β-actin antibody was used at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HepG2 cells. Anti-β-actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in NIH/3T3 cells. Anti-β-actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

    • ICC shows positive staining in C6 cells. Anti-β-actin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

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