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β-tubulin Recombinant Rabbit mAb (SDT-R014)

TUBB,TUBB5,Tubulin beta-5 chain

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B0004
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    β-tubulin

  • 分子别名

    TUBB; TUBB5; Tubulin beta-5 chain

  • 免疫原

    Recombinant Protein

  • 细胞定位

    Intracellular

  • Accession

    P07437

  • 克隆号

    SDT-R014

  • 抗体类型

    Rabbit mAb

  • 反应种属 ?

    Hu, Ms, Rt

  • 纯化方式

    Protein A

  • 浓度

    0.5mg/ml

  • 分子量

    55kDa

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied.

  • 应用

    ICFCM

    IHC-P ?

    ICC ?

    WB

  • 稀释度

    应用 稀释度 推荐种属
    ICFCM 1:500 Hu
    WB 1:1000-1:10000 Hu, Ms, Rt
    IHC-P 1:1000 Hu, Ms, Rt
    ICC 1:500 Hu
背景介绍

  • α- and β-tubulin polymerize into dynamic microtubules. In eukaryotes, microtubules are one of the major components of the cytoskeleton, and function in many processes, including structural support, intracellular transport, and DNA segregation. To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. The β-tubulin subunit is exposed on the plus end of the microtubule, while the α-tubulin subunit is exposed on the minus end. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the β-tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament.

  • 免疫印迹

    • WB result of β-Tubulin Rabbit mAb

      Primary antibody: β-Tubulin Rabbit mAb at 1/1000 dilution
      Lane 1: Hela whole cell lysate 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa

    • WB result of β-Tubulin Rabbit mAb
      Primary antibody: β-Tubulin Rabbit mAb at 1/1000 dilution
      Lane 1:Mouse brain lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg

      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa

    • WB result of β-Tubulin Rabbit mAb

      Primary antibody: β-Tubulin Rabbit mAb at 1/1000 dilution
      Lane 1:rat brain lysate 20 µg


      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa


    • WB result of β-Tubulin Rabbit mAb
      Primary antibody: β-Tubulin Rabbit mAb at 1/10000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: NIH/3T3 whole cell lysate 20 µg
      Lane 3: rat brain lysate 20 µg
      Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 50 kDa
      Observed MW: 52 kDa

  • 流式分析

    • Flow cytometric analysis of HeLa cells labelling β-tubulin antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human testis.

      Anti-β-tubulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human placenta.

      Anti-β-tubulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human cerebral cortex.

      Anti-β-tubulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human colon cancer.

      Anti-β-tubulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse liver.

      Anti-β-tubulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat kidney.

      Anti-β-tubulin antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Counterstained with hematoxylin.

      Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 免疫细胞化学

    • ICC shows positive staining in HeLa cells. Anti-β-tubulin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).

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