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分子别名
Cobalamin binding intrinsic factor, CBLIFAccession
A0A8D1ENZ1表达序列
Protein sequence (A0A8D1ENZ1, Ser27-Try425(S90T, R247Q), with C-His Tag) STQTRSSCSVPSAEQPLVNGIQVLMEQSVTSSAFPNPSILIAMNLAGAYNTEAQELLTYKLMATNTSDLTTGQLALTIMALTSSCRDPGNRIAILQGQMENWAPPSLDTHASTFYEPSLGILTLCQNNPEKTLPLAARFAKTLLANSSPFNMDTGAMATLALTCMYNKIPVGSEEGYRALFSQVLRNTVENISMRIQDNGIIGNIYSTGLAMQALSVTPEQPNKEWDCQKTMDTVLTEIKEGKFHNPMAIAQILPSLKGKTYLDVPHVSCSPGHEVPPTLPNHPSPVPTPAPNITVIYTINNQLRGVELLFNETISVSVKRGSVLLIVLEEAQRKNPKFKFETTMTSWGPVVSSINNIAENVNHRTYWQFLSGQTPLNEGVADYIPFNHEHITANFTQY
表达宿主
HEK293分子量
Predicted MW: 45.4 kDa Observed MW: 50-55 kDa纯度
＞95% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated性状
Lyophilized Powder缓冲体系
Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose.
溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.储存条件
12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
Cobalamin-binding intrinsic factor (CBLIF) is a glycoprotein primarily secreted by gastric parietal cells in pigs, which plays a vital role in cobalamin (vitamin B12) absorption. It binds to dietary cobalamin with high specificity in the stomach, forming a stable complex. This CBLIF-cobalamin complex is resistant to intestinal degradation and is specifically recognized by cubilin receptors on the mucosal surface of the distal ileum, facilitating its active transport into enterocytes. Therefore, CBLIF is essential for the efficient uptake and systemic delivery of vitamin B12, a critical cofactor for DNA synthesis and red blood cell formation.
电泳
2μg（R: reducing conditions）
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