热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 诊断抗原 动物诊断抗原

所有图片（1/1）

ASFV p54, His tag

pE183L

价格 ￥400.00 （ 供应商现货 ： 3-5个工作日）
货号 S0A9002
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    African Swine Fever

  • 分子别名

    pE183L

  • Accession

    Q65194

  • 表达序列

    Protein sequence(Q65194 Ser54-Leu183, with C-10*His)
    SRKKKAAAAIEEEDIQFINPYQDQQWAEVTPQPGTSKPAGATTASAGKPVTGRPATNRPATNKPVTDNPVTDRLVMATGGPAAAPAAASAHPTEPYTTVTTQNTASQTMSAIENLRQRNTYTHKDLENSLGGGGSHHHHHHHHHH

  • 表达宿主

    E.coli

  • 分子量

    Theoretical:15.5kDa Actual: 20kDa

  • 纯度

    >95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 标记

    Unconjugated

  • 性状

    Lyophilized Powder

  • 缓冲体系

    Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, 1mM EDTA, 1mM DTT, pH7.4. Normally trehalose is added as protectant before lyophilization.

  • 溶解方法

    Reconstitute no more than 1mg/mL according to the size in deionized water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, -20 to -70 °C as supplied.
    · 6 months, -20 to -70 °C under sterile conditions after reconstitution.
    · 1 week, 2 to 8 °C under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.

背景介绍

  • African swine fever (ASF) is a highly lethal hemorrhagic viral disease of swine that usually results to a mortality rate approaching 100% in domestic pigs and is classified as a notifiable disease by the World Organization for Animal Health (OIE). A number of studies have reported that p54 is one of the most important ASFV proteins and plays a key role in virus morphogenesis and viral infection. Anti-p54 sera were found to inhibit ASFV attachment to susceptible cells, suggesting its role in virus entry. Similarly, p54/E183L gene plays an essential role in virus viability and recruitment of envelop precursors to assembly sites. Most importantly, E183L gene is crucial for virus particle transporting to perinuclear factory via direct binding to light chain 8 (LC8) of the dynein. A short p54 peptide (149–161 aa) near to its C-terminus is enough to bind to dynein light chain 8 (DLC8) and act as a cargo transport for virus particle.

  • 电泳

    • 2μg (R: reducing conditions)

评论(0)