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Human FSH alpha&FSH beta/CGA&FSHB protein, No tag&His tag

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货号： S0A8011

大包装询价

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产品规格

物种

Human
分子别名

Follicle Stimulating Hormone, Follitropin, FSH
Accession

P01215, P01225
表达序列

Protein sequence1 (P01215, Ala25-Ser116) APDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS;
Protein sequence2 (P01225, Asn19-Glu129, with C-His tag) NSCELTNITIAIEKEECRFCISINTTWCAGYCYTRDLVYKDPARPKIQKTCTFKELVYETVRVPGCAHHADSLYTYPVATQCHCGKCDSDSTDCTVRGLGPSYCSFGEMKE
表达宿主

HEK293
分子量

Predicted MW: 10.2 kDa(CGA)&14.2 kDa(FSHB)；Observed MW: 20-25 kDa
纯度

＞95% by SDS-PAGE
内毒素含量

<0.1EU/μg
标记

Unconjugated
标签

with C-His tag (FSHB)&No tag (CGA)
性状

Lyophilized powder
缓冲体系

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
溶解方法

Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
储存条件

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

背景介绍

Follicle-stimulating hormone (FSH) is a gonadotropin, a glycoprotein polypeptide hormone. FSH is synthesized and secreted by the gonadotropic cells of the anterior pituitary gland and regulates the development, growth, pubertal maturation, and reproductive processes of the body. FSH and luteinizing hormone (LH) work together in the reproductive system. FSH is a 35.5 kDa glycoprotein heterodimer, consisting of two polypeptide units, alpha and beta. The alpha subunits of the glycoproteins LH, FSH, TSH, and hCG are identical and consist of 96 amino acids, while the beta subunits vary. Both subunits are required for biological activity. FSH has a beta subunit of 111 amino acids (FSH β), which confers its specific biologic action, and is responsible for interaction with the follicle-stimulating hormone receptor. The sugar portion of the hormone is covalently bonded to asparagine, and is composed of N-acetylgalactosamine, mannose, N-acetylglucosamine, galactose, and sialic acid.

生物活性
- Immobilized Human FSH alpha&FSH beta/CGA&FSHB protein, No tag&His tag at 2 μg/mL (50 μL/well) can bind FSHB Recombinant Rabbit mAb (SDT-R287) (Cat. No. S0B2272) with EC50 of 22-38 ng/mL.
电泳(SDS-PAGE)
- 2 μg（R: reducing conditions; NR: non-reducing conditions）