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分子量
Predicted MW: 27.4 kDa Observed MW: 27.4 kDa纯度
＞95% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated性状
Lyophilized Powder缓冲体系
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4 with 3% trehalose.
溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.储存条件
12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
2C6 scFv is a recombinant single-chain variable fragment derived from human monoclonal antibody 76-Q3-2C6 isolated from HIV long-term nonprogressors. Constructed by linking the heavy and light variable domains of parental 2C6 antibody via a flexible peptide linker, this small antibody fragment specifically recognizes a conserved, nonlinear conformational epitope on the ectodomain of HIV-1 gp41 across multiple viral clades. It exhibits potent ADCC activity against HIV envelope trimers rather than strong neutralization capacity, and is widely engineered for CAR-T construction, viral detection, and AIDS therapeutic research owing to its low molecular weight, superior tissue penetration and easy prokaryotic/eukaryotic expression.
流式分析
Flow cytometric analysis of 2C6 scfv Protein expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with 2C6 scfv Protein (right panel) at 1ug/test or unlabel (left panel). G4S Linker (E7O2V) Rabbit Monoclonal Antibody conjugated Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were perfommed using Agilent NovoCyte Quanteon and FlowJorM software.
电泳
2μg（R: reducing conditions）
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