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分子量
Predicted MW: 28.4 kDa Observed MW:28.4 kDa
纯度
＞90% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated性状
Lyophilized Powder缓冲体系
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4 with 3% trehalose.
溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.储存条件
12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
C11D5.3 scFv is a recombinant single-chain variable fragment derived from mouse anti-human BCMA IgG1 monoclonal antibody clone C11D5.3, targeting BCMA (CD269) which is highly and selectively expressed on multiple myeloma and malignant B lymphoma cells; constructed by linking the VH and VL domains of the parent antibody via a flexible peptide linker, this small fragment retains robust specific binding to human BCMA and serves as the most widely adopted antigen-recognition extracellular domain for BCMA-targeted CAR-T constructs, including the FDA-approved CAR therapy Abecma, and is recombinantly produced for preclinical immunotherapy research, CAR detection assays and affinity characterization.
流式分析
Flow cytometric analysis of C11D5.3 scfv Protein expression on U266B1(Human multiple myeloma B lymphocyte, Right) cells.The right panel was stained with C11D5.3 scfv Protein at 1ug/test and left panel was unlabel. APC anti-His Tag Antibody was used as the secondary antibody. Flow cytometry and data analysis were perfommed using Agilent NovoCyte Quanteon and FlowJorM software.
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