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分子别名
Phosphatidylethanolamine transferase Mcr-1, Polymyxin resistance protein MCR-1, mcr-1Accession
A0A0R6L508表达序列
Protein sequence (A0A0R6L508, Try179-Arg541, with C-His Tag) YASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYHAKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSCGTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLPKAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAYFKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDVSMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDAITPTLLKLFDVTADKVKDRTAFIR
表达宿主
E.coli分子量
Predicted MW: 42.4 kDa Observed MW: 40 kDa纯度
＞95% by SDS-PAGE内毒素含量
<1EU/μg标记
Unconjugated性状
Lyophilized Powder缓冲体系
Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose.
溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.储存条件
12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
The MCR-1 protein, encoded by the *mcr-1* gene in Escherichia coli and other Enterobacteriaceae, is a phosphoethanolamine transferase. Its primary function is to modify the lipid A component of lipopolysaccharide (LPS) in the bacterial outer membrane by adding phosphoethanolamine. This modification significantly reduces the net negative charge of the membrane, thereby conferring resistance to colistin (polymyxin E), a last-resort antibiotic for treating multidrug-resistant Gram-negative infections. First reported in China in 2015, MCR-1 is notable because its gene is often plasmid-borne, enabling horizontal transfer between bacterial species and facilitating the rapid global spread of colistin resistance, posing a major public health threat.
电泳
2μg（R: reducing conditions）
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