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物种
Human分子别名
Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1(PLAP-1), PLAPAccession
P05187表达序列
Protein sequence (P05187, Ile23-Asp506, with C-His tag) IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD
表达宿主
HEK293分子量
Predicted MW: 54.4 kDa Observed MW: 70 kDa
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
with C-His tag性状
Lyophilized Powder缓冲体系
Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.储存条件
12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.
Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. Alkaline phosphatase, placental type is a membrane-bound glycosylated dimeric enzyme, also referred to as the heat-stable form, that is expressed primarily in the placenta, although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. PLAP is a tumor marker, especially in seminoma and ovarian cancer (e.g., dysgerminoma).
生物活性
Immobilized Human ALPP Protein, His tag at 2 μg/mL (100 μL/well) can bind PLAP+ALPI Recombinant Rabbit mAb (SDT-167-155) (Cat. No. S0B2281) with EC50 of 1.4-1.9 ng/mL.
电泳
2μg(R: reducing conditions)







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