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产品中心 诊断抗原 肿瘤标志物

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Human Glypican-3 , His tag

GTR2-2,Intestinal protein OCI-5,MXR7,GPC3,OCI5

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0A6021
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    GTR2-2, Intestinal protein OCI-5, MXR7, GPC3, OCI5

  • Accession

    P51654

  • 表达序列

    Protein sequence(P51654, Gln25-His559, with C-10*His) QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHGGGGSHHHHHHHHHH

  • 表达宿主

    HEK293

  • 分子量

    Theoretical:39.8kDa Actual: 39, 72-180kDa

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.

  • 溶解方法

    Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles.

背景介绍

  • Glypican-3 is a protein that, in humans, is encoded by the GPC3 gene. The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa. GPC3 has been found to regulate Wnt/β-catenin and Yap signaling pathways. GPC3 interacts with both Wnt and frizzled (FZD) to form a complex and triggers downstream signaling. Glypican 3 immunostaining has utility for differentiating hepatocellular carcinoma (HCC) and dysplastic changes in cirrhotic livers; HCC stains with glypican 3, while liver with dysplastic changes and/or cirrhotic changes does not. GPC3 is also expressed to a lesser degree in melanoma, ovarian clear-cell carcinomas, yolk sac tumors, neuroblastoma, hepatoblastoma, Wilms' tumor cells, and other tumors. However, the significance of GPC3 as a diagnostic tool for human tumors other than HCC is unclear.

  • 生物活性

    • Immobilized Human Glypican-3 , His tag at 1 μg/mL (50 μL/well) can bind Glypican-3 Recombinant Rabbit mAb (SDT-R032) (S0B2111) with EC50 of 4.856-7.279 ng/mL.

  • 电泳

    • 2μg(R: reducing conditions)

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