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物种
SARS-CoV-2分子别名
n/aAccession
P0DTC9表达序列
Protein sequence(P0DTC9, Met1-Ala419, with C-10*His) MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQAGGGGSHHHHHHHHHH
表达宿主
HEK293分子量
Theoretical:47.3kDa Actual:60kDa纯度
＞90% by SDS-PAGE内毒素含量
<1EU/μg标签
His Tag性状
Lyophilized Powder缓冲体系
Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.溶解方法
Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.储存条件
12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles.
Severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2)[2] is a strain of coronavirus that causes COVID-19 (coronavirus disease 2019), the respiratory illness responsible for the ongoing COVID-19 pandemic.The nucleocapsid (N) protein is a protein that packages the coronaviruses to form ribonucleoprotein structures enclosed within the viral capsid. In addition to its interactions with RNA, N forms protein-protein interactions with the coronavirus membrane protein (M) during the process of viral assembly. N also has additional functions in manipulating the cell cycle of the host cell. The N protein is highly immunogenic and antibodies to N are found in patients recovered from SARS and Covid-19.
生物活性
Immobilized SARS-CoV-2 Nucleocapsid Protein , His tag at 1 μg/mL (50 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein Recombinant Rabbit mAb (SDT-021-30) (S0B3009) with EC50 of 5.018-6.825 ng/mL.
电泳
2μg (R: reducing conditions)
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