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产品中心 诊断抗原 CD分子抗原

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Human CD40 Ligand/TNFSF5 protein, His tag

CD40 ligand,CD40-L,T-cell antigen Gp39,TNF-related activation protein (TRAP),Tumor necrosis factor ligand superfamily member 5,CD154,CD40LG,CD40L,TNFSF5,TRAP

价格 ￥900.00 （ 供应商现货 ： 3-5个工作日）
货号 S0A1123
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein (TRAP), Tumor necrosis factor ligand superfamily member 5, CD154, CD40LG, CD40L, TNFSF5, TRAP

  • Accession

    P29965

  • 表达序列

    Protein sequence (P29965, Met113-Leu261, with C-His tag) MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

  • 表达宿主

    HEK293

  • 分子量

    Predicted MW: 17.9 kDa Observed MW: 18, 19 kDa

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    with C-His tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.

  • 溶解方法

    Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied.
    6 months, -20 to -70 °C under sterile conditions after reconstitution.
    1 week, 2 to 8 °C under sterile conditions after reconstitution.
    Please avoid repeated freeze-thaw cycles.

背景介绍

  • CD154, also called CD40 ligand, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and integrin αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells (TFH cells). On TFH cells, CD154 promotes B cell maturation and function by engaging CD40 on the B cell surface and therefore facilitating cell-cell communication. A defect in this gene results in an inability to undergo immunoglobulin class switching and is associated with hyper IgM syndrome. Absence of CD154 also stops the formation of germinal centers and therefore prohibiting antibody affinity maturation.

  • 生物活性

    • Immobilized Biotinylated CD40 Fc&Avi Tag, Human (Cat. No. UA010443) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Human CD40 Ligand/TNFSF5 protein, His tag with EC50 of 1.20-2.85ng/ml.

  • 电泳

    • 2 μg（R: reducing conditions）

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