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产品中心 药物靶点和Fc受体 小分子药物靶点蛋白

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Human KRAS Protein, His Tag

GTPase KRas,K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras,KRAS2,RASK2,K-Ras4B

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货号 S0A0230
规格
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产品介绍

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产品规格

  • 物种

    Human

  • 分子别名

    GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, KRAS2, RASK2, K-Ras4B

  • Accession

    P01116-2

  • 表达序列

    Protein sequence (P01116-2, Thr2-Cys185, with C-His Tag) TEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKC

  • 表达宿主

    E.coli

  • 分子量

    Predicted MW: 22.8 kDa Observed MW: 22.8 kDa

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 活性

    The specific activity of KRAS His Tag Protein, Human was determined to be > 600 pmol/min/mg in a GTPase-Glo assay using GTP solution substrate.

  • 标记

    Unconjugated

  • 性状

    Lyophilized Powder

  • 缓冲体系

    Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4 with 3% trehalose.

  • 溶解方法

    Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied.
    6 months, -20 to -70 °C under sterile conditions after reconstitution.
    1 week, 2 to 8 °C under sterile conditions after reconstitution.
    Please avoid repeated freeze-thaw cycles.

背景介绍

  • GTPase KRas, a product of the KRAS oncogene, is a molecular switch that cycles between inactive GDP-bound and active GTP-bound states to regulate cell proliferation. Upon growth factor stimulation, it activates downstream pathways like MAPK and PI3K-AKT. Its activity is tightly controlled by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). However, cancer-associated point mutations at codons 12, 13, or 61 impair GTP hydrolysis, locking KRas in a constitutively active state that drives uncontrolled cell growth.

  • 电泳

    • 2μg（R: reducing conditions）

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