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E. coli MBP Protein, His tag

Maltose/maltodextrin-binding periplasmic protein,MMBP,Maltodextrin-binding protein,Maltose-binding protein (MBP),malE

价格 ￥1,100.00 （ 供应商现货 ： 3-5个工作日）
货号 S0A0132
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    E. coli

  • 分子别名

    Maltose/maltodextrin-binding periplasmic protein, MMBP, Maltodextrin-binding protein, Maltose-binding protein (MBP), malE

  • Accession

    P0AEX9

  • 表达序列

    Protein sequence (P0AEX9, Lys27-Thr392, with C-His tag) KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT

  • 表达宿主

    E.coli

  • 分子量

    Predicted MW: 41.8 kDa Observed MW: 42 kDa

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    with C-His tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.

  • 溶解方法

    Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.

  • 储存条件

    12 months from date of receipt, -20 to -70 °C as supplied.
    6 months, -20 to -70 °C under sterile conditions after reconstitution.
    1 week, 2 to 8 °C under sterile conditions after reconstitution.
    Please avoid repeated freeze-thaw cycles.

背景介绍

  • Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. The fusion of proteins with MBP usually enhances their solubility and facilitates their proper folding. In addition, such fusions can facilitate the crystallization of difficult proteins, e.g. membrane proteins. MBP can itself be used as an affinity tag for purification of recombinant proteins.

  • 电泳

    • 2 μg（R: reducing conditions）

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