Human Proprotein convertase 9 (PCSK9), His tag

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货号： S0A0033

Datasheet COA

大包装询价

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产品规格

物种

Human
分子别名

Proprotein convertase subtilisin/kexin type 9, Neural apoptosis-regulated convertase 1 (NARC-1), PC9, Subtilisin/kexin-like protease PC9
Accession

Q8NBP7
表达序列

Protein sequence(Q8NBP7, Gln31-Gln692, with C-10*His) QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVVVLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSDCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCRSRHLAQASQELQGGGGSHHHHHHHHHH
表达宿主

HEK293
分子量

Theoretical:72.7kDa Actual:65kDa
纯度

＞95% by SDS-PAGE
内毒素含量

<1EU/μg
标签

His Tag
性状

Lyophilized powder
缓冲体系

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4.
溶解方法

Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
储存条件

12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles.

背景介绍

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is the 9th member of the proprotein convertase family of proteins that activate other proteins. PCSK9 is ubiquitously expressed in many tissues and cell types. PCSK9 binds to and degrades the receptor for low-density lipoprotein particles (LDL), which typically transport 3,000 to 6,000 fat molecules (including cholesterol) per particle, within extracellular fluid.Recent evidence indicate that PCSK9 is highly expressed in arterial walls such as endothelium, smooth muscle cells, and macrophages, with a local effect that can regulate vascular homeostasis and atherosclerosis.

生物活性
- Immobilized Human Proprotein convertase 9 (PCSK9), His tag at 1 μg/mL (50 μL/well) can bind Proprotein convertase 9/PCSK9 Recombinant Rabbit mAb (SDT-424-113) (Cat. No. S0B0234) with EC50 of 2.938-3.598 ng/ ml.
电泳(SDS-PAGE)
- 2μg (R: reducing conditions)