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产品中心 药物靶点和Fc受体 小分子药物靶点蛋白

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CRBN/DDB1/CUL4A/RBX1 Protein, Human

价格 ￥3,540.00 （ 1-2周）
货号 UA080275
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • Accession

    Q96SW2, Q16531, Q13619, P62877

  • 表达序列

    M1-L442 end(CRBN)
    M1-H1140 end(DDB1)
    A2-A759 end(Cul4a)
    M1-H108 end(RBX1)
    N-His-TEV-Avi (CRBN)/No tag (DDB1)/ No tag (Cul4a)/No tag
    (RBX1)

  • 表达宿主

    Baculovirus-InsectCells

  • 分子量

    55.3 KDa/126.9 KDa/87.6 KDa/12.2 KDa

  • 标记

    Unconjugated

  • 标签

    Avi Tag, His Tag

  • 性状

    Liquid

  • 缓冲体系

    50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT

  • 储存条件

    -80℃

注意事项

  • Please use rapid thawing with running water to thaw the protein samples.

    For complete recovery, mix well and spin before use.

    Product must not be stored in diluted solutions.

    Aliquots below 10μl are not advisable.

    Avoid repetitive freeze-thaw cycles!

  • 生物活性

    • Lane M: Marker Lane 1: Bodipy-Ub + UBE1 + Ubch5b + CRBN/DDB1/CUL4A/RBX1 with ATP
      Lane 2: Bodipy-Ub + UBE1+ Ubch5b with ATP
      Lane 3: Bodipy-Ub + UBE1+ Ubch5b without ATP
      The ubiquitin conjugating activity of CRBN/DDB1/CUL4A/RBX1 was validated through its ability to catalyse
      the generation of polyubiquitin chains in the presence of the E1 activating enzyme UBE1, the E2 conjugating
      enzyme Ubch5b and Bodipy-ubiquitin. Incubation of CRBN/DDB1/CUL4A/RBX1 for 60 minutes at 37˚C in the
      presence of Bodipy-ubiquitin, UBE1, Ubch5b and ATP (Lane 1) was compared alongside two control reactions
      with either CRBN/DDB1/CUL4A/RBX1 (Lane 2) or CRBN/DDB1/CUL4A/RBX1&ATP (Lane 3) excluded from
      the reaction. Ubiquitin conjugates were identified by the migration of the Bodipy-ubiquitin band and these were
      observed only in the presence of ATP and CRBN/DDB1/CUL4A/RBX1.

  • 电泳

    • Lane M: Marker
      Lane 1: 2 μg, reduced
      Lane 2: 2 μg, non-reduced

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