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产品规格
物种
Human分子别名
DDB1/CRBN ComplexAccession
Q16531-1, Q96SW2-1表达序列
CRBN(M1-L442 end) DDB1(M1-H1140 end)N-terminal 6x His tag
表达宿主
Baculovirus-InsectCells分子量
53.7 KDa/126.9 KDa
纯度
>85% by SDS-PAGE标记
Unconjugated标签
His Tag性状
Liquid缓冲体系
50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5
储存条件
Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
生物活性
The CRBN/DDB1 activity was detected using HTRF technology. The reaction was performed by incubating the varying concentration of lenalidomide, CRBN/DDB1 protein, substrate and beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
电泳
M MW Standard
1 3 μg CRBN/DDB1
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