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Recombinant Human α-Chymotrypsin, Mass Spectrometry Grade

α-chymotrypsin A and B,alpha-chymotrypsin

价格 ￥1,760.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070117
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    α-chymotrypsin A and B, alpha-chymotrypsin

  • 表达宿主

    Pichia pastoris

  • 分子量

    27 kDa (Reducing)

  • 纯度

    ＞98.7% by SDS-PAGE and HPLC

  • 活性

    ≥200 U/mg

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    1 mM HCl, 2 mM CaCl2, pH 3.0

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    1. Simon LM, Kotormán M, Garab G, Laczkó I. Structure and activity of alpha-chymotrypsin and trypsin in aqueous organic media. Biochem Biophys Res Commun. 2001 Feb 9;280(5):1367-71. PMID: 11162681.
    2. Pham VT, Ewing E, Kaplan H, Choma C, Hefford MA. Glycation improves the thermostability of trypsin and chymotrypsin. Biotechnol Bioeng. 2008 Oct 15;101(3):452-9. PMID: 18470893.

背景介绍

  • Chymotrypsin is a serine endoproteinase that specifically cleaves peptide bonds at the C-termini of Tyr, Phe, Trp and Leu. Met, Ala, Asp and Glu may be cleaved at a much lower rate.

产品组分

  • Recombinant α-Chymotrypsin, human lyophilized powder

操作步骤

  • Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
    Add Recombinant α-Chymotrypsin, human, to a final protease: protein ratio of 1:200 to 1:20(w/w), and incubate sample for 2–18 hours at 25°C. The reaction may be stopped, if desired, by adding 0.5% trifluoroacetic acid.

酶活定义
  • One unit is defined as the amount of Chymotrypsin that will hydrolize 1.0µmol of BTEE per minute at pH 7.8 and 25°C.

  • 生物活性

    • In the experimental design, BTEE substrate was prepared, and 6 ul α-chymotrypsin of 0.2 mg/mL was added to the uartz cuvette with an optical path of 1cm of 2994 ul substrate and the absorption value of 256 nm was read every 10 seconds at 25 ℃.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • 98.7%

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