分享产品介绍 评论(0)
物种
PIG分子别名
Trypsin(pig); porcine pancreas; Proenzyme; Parenzymol; Pseudotrypsin ;Tryptar; Trypure;浓度
0.15mg/ml分子量
24 kD
纯度
＞95% by SDS-PAGE活性
830 USP/ml标签
No Tag性状
Liquid缓冲体系
0.9% sodium chloride, pH 7.0储存条件
12 month, 2 to 8 °C under sterile conditions
Store at -20℃ and avoid repeated freeze-thaw cycles.文献引用
[1] Charles M , Rovery M , Guidoni A ,et al.Trypsinogen and trypsin of pig[J].Biochimica et Biophysica Acta, 1963, 69:115-129.
[2] Keryn,Dallas,Johnson,et al.A functional comparison of ovine and porcine trypsins[J].Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology, 2002.
Trypsin (EC3.4.21.4) is part of the serine protease family. Trypsin cleaves lysine and arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site and no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. The stringent specificity of trypsin is essential for protein identification, and it has become the gold standard for protein digestion to peptides for shotgun proteomics. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester and amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester (BAEE), p-toluenesulfonyl- L-arginine methyl ester (TAME), tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide (BAPNA), L-lysyl-p-nitroanilide, and benzoyl-L-tyrosine ethyl ester (BTEE). Serine protease inhibitors that inhibit recombinant trypsin include TLCK (N-p-tosyl-L-lysine chloromethyl ketone), PMSF (phenylmethanesulfonyl fluoride), benzamidine, soybean trypsin inhibitor, and ovomucoid.
1x, 0.15 g Recombinant porcine trypsin and 0.02 g EDTA per liter of 0.9% sodium chloride
The time required to remove cells from the culture surface is dependent on cell type, population density, serum concentration in the growth medium, potency of trypsin, and time since last subculture. Trypsin can cause cellular damage, thus time of exposure should be kept to a minimum.
- In a 3.2ml reaction system using BAEE as substrate, the UV absorption at 253nm increased by 0.003 per min using cuvette with 1 cm optical path, which was defined as 1 USP trypsin activity unit
生物活性
In the experimental design, 0.25 mM BAEE substrate was prepared, and 10 ul Trypsin of 0.54 mg/mL was added to the uartz cuvette with an optical path of 1cm of 3000 ul substrate and 190 ul buffer, and the absorption value of 253 nm was read every 30 seconds at 25 ℃.
Dispense enough Trypsin Solution into culture vessel(s) to completely cover the monolayer of cells and place in RT incubator for 3 minutes.
电泳
2μg (R: reducing condition).
反相高效液相色谱(RP-HPLC)
评论(0)