热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 酶制剂 多肽测序

所有图片（1/4）

Endoproteinase Glu-C, Mass Spectrometry Grade

Glutamyl endopeptidase、V8 protease、Endoproteinase Glu-C、V8 proteinase、V8、Glu-C

价格 ￥780.00 （ 供应商现货 ： 3-5个工作日）
货号 UA070059
规格
数量
收藏 分享

产品介绍 评论(0)

产品规格

  • 物种

    Staphylococcus aureus

  • 分子别名

    Glutamyl endopeptidase、V8 protease、Endoproteinase Glu-C、V8 proteinase、V8、Glu-C

  • 表达宿主

    E.coli

  • 分子量

    25kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 标签

    His Tag

  • 性状

    Lyophilized powder

  • 溶解方法

    To get the most use out the enzyme, resuspend the enzyme in 500 μl H2O and aliquot 50 ul each in 10 tubes.

  • 储存条件

    Store at -25 ~ -15℃ for 2 years

  • 文献引用

    [1] Yabuta M , Ochi N , Ohsuye K .Hyperproduction of a recombinant fusion protein of Staphylococcus aureus V8 protease in Escherichia coli and its processing by OmpT protease to release an active V8 protease derivative.[J].Appl Microbiol Biotechnol, 1995, 44(1-2):118-125.
    [2] Prasad L , Leduc Y , Hayakawa K ,et al. The structure of a universally employed enzyme: V8 protease from Staphylococcus aureus[J].Acta Crystallographica Section D Biological Crystallography, 2004.

背景介绍

  • Staphylococcus aureus V8 protease (Endoproteinase Glu-C) belongs to the serine protease family and can specifically hydrolyze carboxy-terminal peptide bonds of glutamic acid (Glu) or aspartate (Asp) residues. Its specificity is directly affected by the composition of the buffer, recognizing and cutting Glu carboxy-terminal peptide bonds in NH4HCO3 of pH7.8 and CH3COONH4 of pH4.0, recognizing and cutting Glu or Asp carboxy-terminal peptide bonds in phosphate buffers of pH7.8，but the hydrolysis rate of Glu was higher than that of Asp. Glu-C has been reported to be active in 0.2% SDS (sodium lauryl sulfate) and 4.0M urea.

产品组分

  • Supplied in dry format from Tris-HCl and sodium chloride buffer

操作步骤

  • For the digestion of peptides or proteins, the ratio of enzyme to substrate between is 1:20 and 1:100 (w/w) for recommended. Dissolve the peptide or protein and was enzymolysis in 100 mM NH4HCO3, pH 7.8 or 100 mM Tris-HCl, pH 7.8, the recommended incubation time is 2-18 hours at 37°C, depending on the temperature and the ratio of enzyme to substrate.

注意事项

  • Repeated freeze/thawing is not recommended.

酶活定义
  • Using 2-Ph-leu-Glu-4-Na as substrate, the amount of enzyme required to produce 1μmol 4-Nitroaniline in 1 minute at pH7.8, 25℃ was 1unit.

  • 生物活性

    • Bovine Serum Albumin样品经Glu-C酶解及LC-MS/MS分析后序列覆盖率为98.3%

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

评论(0)