H1N1 NP (A/Puerto Rico/8/1934) His Tag Protein, Influenza

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货号： UA030056

Datasheet COA

价格： ￥3,020
规格：
100μg
数量：

大包装询价

产品介绍引用文献(0) 评论(0)

产品规格

物种

Influenza
抗原名称

NP/Nucleoprotein
分子别名

Nucleoprotein, Nucleocapsid protein, NP.
Accession

Accession#: P03466
表达序列

Met1-Asn498, with C-terminal 10*His MASQGTKRSYEQMETDGERQNATEIRASVGKMIGGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRVNGKWMRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELVRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQKAMMDQVRESRNPGNAEFEDLTFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVLSFIKGTKVLPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPFDRTTIMAAFNGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKAASPIVPSFDMSNEGSYFFGDNAEEYDNGGGSGGGSHHHHHHHHHH.
表达宿主

Hi5(Baculovirus-InsectCells)
分子量

57-60 kDa
纯度

＞95% by SDS-PAGE and HPLC
内毒素含量

<0.1EU/μg
标记

Unconjugated
标签

His Tag
性状

Lyophilized powder
缓冲体系

1*PBS, 1mM DTT, 1mM EDTA, pH7.4
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用

1.Pang B, Cheung NN, Zhang W, Dai J, Kao RY, Zhang H, Hao Q. Structural Characterization of H1N1 Nucleoprotein-Nucleozin Binding Sites. Sci Rep. 2016 Jul 11;6:29684.
2.Ortega J, Martín-Benito J, Zürcher T, Valpuesta JM, Carrascosa JL, Ortín J. Ultrastructural and functional analyses of recombinant influenza virus ribonucleoproteins suggest dimerization of nucleoprotein during virus amplification. J Virol. 2000 Jan;74(1):156-63.

背景介绍

Nucleoprotein, encoded by influenza A virus, is an attractive target due to its high conservative and multifunctional nature during virus life cycle. In addition, NP does not have any counterpart in the cell, and hence compounds targeting NP might have less off-target side effects. NP encapsulates the negative strand viral RNA by is binding to 24 bases of RNA, protecting it from nucleases. The encapsulated genomic RNA is termed the ribonucleoprotein (RNP). The active RNP imports into the nucleus through cellular importin α/β pathways, and serves as template for transcription and replication.