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物种
Cynomolgus分子别名
ROR1, NTRKR1Accession
Q01973-1表达序列
Glu39-Gly151, with C-terminal His表达宿主
HEK293分子量
25-32kDa (Reducing)
纯度
＞95% by SDS-PAGE & SEC-HPLC标记
Unconjugated标签
His Tag性状
Lyophilized powder缓冲体系
PBS, PH7.4, 5% trehalose溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Mouawad N, Ruggeri E, Capasso G, Martinello L, Visentin A, Frezzato F, Trentin L. How receptor tyrosine kinase-like orphan receptor 1 meets its partners in chronic lymphocytic leukemia. Hematol Oncol. 2024 Mar;42(2):e3250.
Zhang Y, Fan Y, Liu S, Guan Y, Wan J, Ren Q, Wang J, Zhong L, Hu Z, Shi W, Qian H. Development of Peptide Paratope Mimics Derived from the Anti-ROR1 Antibody and Long-Acting Peptide-Drug Conjugates for Targeted Cancer Therapy. J Med Chem. 2024 Jul 11;67(13):10967-10985.
Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.
电泳(SDS-PAGE)
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
The purity of ROR1 (39-151, Ig-like Domain) His Tag Protein, Human/Cynomolgus is more than 95% determined by SEC-HPLC.
ELISA
Immobilized ROR1 (39-151, Ig-like Domain) His Tag Protein, Human/Cynomolgus (Cat. No. UA011055) at 2.0μg/mL (100μL/well) can bind Anti-Human ROR1 (Zilovertamab) with EC50 of 0.92-1.21ng/mL.
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