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Monoclonal Anti-MMAE&MMAF Antibody

价格 ￥1,500.00 （ 供应商现货 ： 3-5个工作日）
货号 S0E0007
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Mouse

  • 反应种属 ?

    Species Independent

  • 浓度

    2 mg/ml

  • 纯度

    >95% (Determined by SDS-PAGE)

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS pH7.4

  • 储存条件

    2 to 8 °C for 2 weeks under sterile conditions;
    -20 °C for 3 months under sterile conditions;
    -80 °C for 24 months under sterile conditions.
    Please avoid repeated freeze-thaw cycles.

  • 应用

    ELISA

  • 验证数据

    • ELISA analysis specificity of MMAE&MMAF mAb
      Coating ADC: 1μg/ml
      Add anti-MMAE&MMAF mAb (biotin conjugated): from 1μg/ml, 3-fold diluent, 7 points
      Add SA-HRP: 1:10000

    • Sandwich ELISA assay
      Coating anti-MMAE&MMAF mAb: 5μg/ml
      Sample: Disitamab vedotin (MMAE ADC) and Belantamab mafodotin (MMAF ADC), from 12.5ng/ml, 2-fold diluent, 7 points
      Add anti human Fc HRP: 1:4000

  • 电泳

    • Fig.1. SDS-PAGE analysis of MMAF-1-88H4
      Lane M: Precision Plus Protein™ Dual Color Standards (Bio-Rad) (Sample loaded: 5 μl)
      Lane 1: Purified MMAF-1-88H4 under reducing condition (Sample loaded: 2 μg)
      Lane 2: Purified MMAF-1-88H4 under non-reducing condition (Sample loaded: 2 μg)
      • Recombinant protein was visualized by Coomassie Brilliant Blue R250 staining.

  • 体积排阻色谱(SEC-HPLC)

    • Fig. 2. SEC-HPLC analysis of MMAF-1-88H4
      Running buffer: 2*PBS, pH 7.4
      Column: Superdex 200 Increase 5/150 GL(GE, Code Number: 28-9909-45)

  • ELISA

    • Fig.4. Sandwich ELISA analysis of MMAF-1-88H4 (CP2023013101)
      Coating Her2 ECD protein: 2ug/ml
      Add MMAF/MMAE-ADC: from 10ug/ml, 10-fold diluent, 8 points including 0
      Add MMAF antibody 88H4 (mouse IgG) at 1ug/ml
      Add anti-mouse IgG (Fc specific)-HRP: 1:5000

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