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储存条件
Store columns dry at +10 to +35 °C.
分选能力
1x108 magnetically labeled cells in 2x109 total cells. Recommended sample size for leukocytes: 105 - 108 labeled cells in 107 - 2x109 total cells. Note: Column capacity may decrease when separating cells larger than lymphocytes.
分选方式
有柱分选
The column Technology is based on the use of NanoBeads, Columns, and Separators. Separation have been developed for the gentle isolation of NanoBead-labeled cells. As NanoBeads are extremely small, superparamagnetic particles, a high-gradient magnetic field is required to retain the labeled cells. Separation contains an optimized matrix to generate this strong magnetic field when placed in a permanent magnet, such as the Starter MagSep Separator (L) or Starter MagSep Separator (4L).
25 L Separation Columns and plungers, sterile packed
验证数据
Use the L separation column to enrich or deplete CD3+ T cells from Jurkat (Human T cell leukemia T lymphocyte, Right) or HL-60 (Human acute promyelocytic leukemia cell line, Left). CD3+ cells were stained with anti-CD3 (clone: UCHT1) Alexa Fluor™ 647 and gated on Live+.
Beads: CD8 Nanobeads, mouse(S0K0005)
Column: L separation column(S0D3011)
图例
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