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抗原名称
IFN-α免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Mk纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8'C as supplied.
Precision
Intra-assay: 7.0%;
Inter-assay: 7.8%Sample type
Cell culture supernatant; Serum; PlasmaAssay type
Sandwich (quantitative)Sensitivity
1.350 pg/mLRange
3.125 pg/mL – 200 pg/mLRecovery
Cell culture supernatant: 108%
Serum: 103%
Plasma: 96%Assay time
60 minutesSpecies reactivity
Mk
IFN-α is a type of cytokine belonging to the Type I interferon family, primarily produced by leukocytes like plasmacytoid dendritic cells. Its main role is as a crucial component of the innate immune system, acting as a first line of defense against viral infections. Upon binding to its receptor (IFNAR), it triggers the JAK-STAT signaling pathway inside the cell. This activation leads to the expression of hundreds of interferon-stimulated genes (ISGs). These genes create an "anti-viral state" by inhibiting various stages of the viral life cycle. Due to this potent anti-viral and immunomodulatory activity, synthetic IFN-α has been developed as a therapeutic drug. It is clinically used to treat several conditions, including hepatitis B and C, as well as certain cancers like hairy cell leukemia.
ELISA
Standard curve
Example of Monkey IFN-α standard curve in Assay Diluent DA4.Linearity
The concentrations of Monkey IFN-α were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 1ug/ml poly I:C for 24 hours (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 10.68 pg/mL in stimulated monkey PBMC supernatant.To assess the linearity of the assay, three samples were spiked with high concentrations of Monkey IFN-α in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram







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