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Monkey IFN-α OneStep ELISA Kit

价格 3,000.00 1-2周
货号 S0C3104
规格
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产品介绍 评论(0)

产品规格
  • 抗原名称

    IFN-α
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Mk
  • 纯化方式

    Protein A
  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数
    • Precision

      Intra-assay: 7.0%;
      Inter-assay: 7.8%
    • Sample type

      Cell culture supernatant; Serum; Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      1.350 pg/mL
    • Range

      3.125 pg/mL – 200 pg/mL
    • Recovery

      Cell culture supernatant: 108%
      Serum: 103%
      Plasma: 96%
    • Assay time

      60 minutes
    • Species reactivity

      Mk
背景介绍
  • IFN-α is a type of cytokine belonging to the Type I interferon family, primarily produced by leukocytes like plasmacytoid dendritic cells. Its main role is as a crucial component of the innate immune system, acting as a first line of defense against viral infections. Upon binding to its receptor (IFNAR), it triggers the JAK-STAT signaling pathway inside the cell. This activation leads to the expression of hundreds of interferon-stimulated genes (ISGs). These genes create an "anti-viral state" by inhibiting various stages of the viral life cycle. Due to this potent anti-viral and immunomodulatory activity, synthetic IFN-α has been developed as a therapeutic drug. It is clinically used to treat several conditions, including hepatitis B and C, as well as certain cancers like hairy cell leukemia.

  • ELISA

    • Standard curve
      Example of Monkey IFN-α standard curve in Assay Diluent DA4.

    • Linearity
      The concentrations of Monkey IFN-α were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Monkey PBMC cells stimulated with 1ug/ml poly I:C for 24 hours (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 10.68 pg/mL in stimulated monkey PBMC supernatant.

    • To assess the linearity of the assay, three samples were spiked with high concentrations of Monkey IFN-α in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Protocol Diagram

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