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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human MCP-1/CCL2 OneStep ELISA Kit

CCL2,MCP-1

价格 ￥2,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3064
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    CCL2

  • 分子别名

    CCL2;MCP-1

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.8%；
      Inter-assay: 3.7%

    • Sample type

      Cell culture supernatant, Serum,Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.692 pg/ml

    • Range

      3.125 pg/mL – 200 pg/mL

    • Recovery

      Cell culture supernatant: 101%
      Serum: 102%
      Plasma: 87%

    • Assay time

      60 minutes

    • Species reactivity

      Human
背景介绍

  • MCP-1, also known as CCL2, is a chemokine belonging to the C-C chemokine family. It plays a crucial role in various physiological processes such as inflammation, immune regulation, and cell migration. MCP-1/CCL2 primarily functions by binding to the C-C chemokine receptor CCR2, thereby guiding monocytes and other inflammatory cells to migrate to sites of inflammation, contributing to the inflammatory response and immune regulation. Overexpression of MCP-1/CCL2 is closely associated with the pathogenesis and progression of various diseases, including inflammatory diseases, autoimmune disorders, cancer, and metabolic disorders. As a result, MCP-1/CCL2 has emerged as a potential therapeutic target, and researchers are exploring therapeutic strategies targeting MCP-1/CCL2 in the hope of impacting the treatment of related diseases.

  • ELISA

    • Standard curve
      Example of Human MCP-1 standard curve in Assay Diluent #11.

    • Linearity
      The concentrations of MCP-1were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Human Serum was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 218 pg/mL in Human Serum.
      #2 sample is undiluted samples are as follows: Human Plasma was 1.3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 103 pg/mL in Human Plasma.

    • Linearity
      The concentrations of MCP-1 were measured and interpolated from the target standard curves and corrected for sample dilution.
      The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 1days (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 8690 pg/mL in stimulated human PBMC supernatant.

    • Linearity
      The sample is undiluted samples are as follows: human PBMC cells culture for 1days (4%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2930 pg/mL in unstimulated human PBMC supernatant.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Human MCP-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, the samples were spiked with high concentrations of Human MCP-1 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Leading Competitor comparison

    • Protocol Diagram

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