产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
IL-10免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Precision
Intra-assay: 2.9%；
Inter-assay:5.6%Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
4.013pg/mlRange
9.38 pg/mL – 600 pg/mLRecovery
Cell culture supernatant: 101%
Serum: 81%
Plasma:85%Assay time
60 minutesSpecies reactivity
HumanNIBSC convert
The dose response curve of the International Standard (93/722) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (93/722) units, use the following equation: NIBSC (93/722) approximate value (IU/mL) = 0.005 x OneStep Human IL-10 value (pg/mL).
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type-II T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 can be produced by monocytes upon PD-1 triggering in these cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.
ELISA
Standard curve
Example of NIBSC (93/722) standard curve in Assay Diluent #12.Standard curve
Example of human IL-10 standard curve in Assay Diluent #12.Linearity
The concentrations of Human IL-10 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 2days (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4895 pg/mL in stimulated human PBMC supernatant.Linearity
The sample is undiluted samples are as follows: human PBMC cells culture for 2days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 58.81pg/mL in unstimulated human PBMC supernatant.To assess the linearity of the assay, three samples were spiked with high concentrations of Human IL-10 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Leading Competitor comparison
Protocol Diagram
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