产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IFN-γ OneStep ELISA Kit

货号: S0C3035

  • 价格: ¥3,200
  • 规格:
  • 数量:
斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格
  • 抗原名称

    IFN-γ
  • 免疫原

    Recombinant Protein
  • 抗体类型

    Recombinant mAb
  • 反应种属 ?

    Ms
  • 纯化方式

    Protein A
  • 储存条件

    2 to 8 °C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数
    • Precision

      Intra-assay: 3.6%;
      Inter-assay: 6.0%
    • Sample type

      Cell culture supernatant, Serum
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      7.398pg/ml
    • Range

      31.25 pg/mL – 2000 pg/mL
    • Recovery

      Cell culture supernatant: 83%
      Serum: 107%
    • Assay time

      60 minutes
    • Species reactivity

      Mouse
背景介绍
  • Mouse IFN-γ, or Interferon-gamma, is a crucial cytokine primarily produced by natural killer (NK) cells and T lymphocytes, particularly Th1 cells and cytotoxic T cells. It plays a pivotal role in the immune response, acting as a key mediator in both innate and adaptive immunity. IFN-γ is known for its potent antiviral, immunoregulatory, and anti-tumor properties. It enhances the presentation of antigens by upregulating major histocompatibility complex (MHC) class I and II molecules on antigen-presenting cells, thereby facilitating a more robust immune response. Additionally, IFN-γ activates macrophages, increasing their ability to phagocytose and destroy pathogens. It also promotes the differentiation of naive T cells into Th1 cells, further amplifying the immune response. In research, mouse IFN-γ is widely used to study immune responses, inflammatory diseases, and host-pathogen interactions, providing valuable insights into the mechanisms of immune regulation and potential therapeutic strategies.

  • ELISA

    • Standard curve
      Example of Mouse IFN-γ standard curve in Assay Diluent #9.

    • Linearity
      The concentrations of IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is  undiluted samples are as follows:  CD-1 serum、BALB/c serum and C57BL/6 serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 111.135 pg/mL in C57BL/C serum.

    • Linearity
      The concentrations of IFN-γ were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is  undiluted samples are as follows:  CD-1 serum、BALB/c serum and C57BL/6 serum(100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 111.135 pg/mL in C57BL/C serum.

    • Protocol Diagram

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