产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
TNF-α免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Ms纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
Intra-assay: 3.8%；
Inter-assay: 5.8%
Sample type
Cell culture supernatant, Serum, PlasmAssay type
Sandwich (quantitative)Sensitivity
0.095 ng/mlRange
0.156 ng/mL – 10 ng/mLRecovery
Cell culture supernatant: 87%
Serum: 106%
Plasm: 105%Assay time
60 minutesSpecies reactivity
MouseCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
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Tumor necrosis factor α (TNF alpha) is an effective pro-inflammatory cytokine, which can kill and inhibit tumor cells. It is mainly produced by activated macrophages and can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neuronal tissues. The members of TNF alpha family exert their cellular effect through two distinct surface receptors of the TNF receptor family, TNFR1and TNFR2. The pleiotropic biological effects of TNF can be attributed to its ability to simultaneously activate multiple signaling pathways in cells. TNF-induced NF-κB activation promotes the growth, invasion and metastasis of cancer cells.
ELISA
Standard curve
Example of Mouse TNF-α standard curve in Assay Diluent #1.
Linearity
The concentrations of TNF-α were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: RAW264.7 mouse monocyte/macrophage cells stimulated with 5ug/ml LPS and 100ng/mL rmIL-10 for 7days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.19 ng/mL in stimulated RAW264.7 supernatant.
Protocol Diagram
引用文献(1)
- Abnormal iron metabolism in the zona incerta in Parkinson's disease mice
M Xiu, Y Liu, Z Wang, J Zhang, Y Shi, J Xie
Journal of Neural Transmission. 2025 Mar 09 .
影响因子： 3.2
货号：S0C3023产品名称：Mouse TNF-α OneStep ELISA Kit
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