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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IL-6 OneStep ELISA Kit

货号： S0C3019

Datasheet COA
  • 价格： ￥3,000
  • 规格：
  • 数量：
斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-6

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    2 to 8 °C as supplied.

  • 稀释度

    [{"application": "ELISA", "dilution": "", "species": ""}, {"application": "Sandwich ELISA", "dilution": "", "species": ""}, {"application": "CLIA", "dilution": "", "species": ""}, {"application": "Lateral Flow", "dilution": "", "species": ""}, {"application": "Dot Blot", "dilution": "", "species": ""}, {"application": "WB", "dilution": "", "species": ""}, {"application": "IP", "dilution": "", "species": ""}, {"application": "IHC-P", "dilution": "", "species": ""}, {"application": "ICC", "dilution": "", "species": ""}, {"application": "IF", "dilution": "", "species": ""}, {"application": "ICFCM", "dilution": "", "species": ""}, {"application": "FCM", "dilution": "", "species": ""}, {"application": "mIHC", "dilution": "", "species": ""}]
试剂盒参数

    • Precision

      板内变异系数（Intra-assay）：4.1%；
      板间变异系数（Inter-assay）：5.1%

    • Sample type

      Cell culture supernatant, Serum, Plasm,  Cell/Tissue Lysate 

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      2.788 pg/ml

    • Range

      7.81 pg/mL - 500 pg/mL

    • Recovery

      Cell culture supernatant: 102%
      Serum: 106%
      Plasm: 102%

    • Assay time

      60 minutes

    • Species reactivity

      Mouse
背景介绍

  • Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. Mouse IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 in regulating the acute phase response. Mouse IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 has a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.

  • ELISA

    • Standard curve
      Example of Mouse IL-6 standard curve in Assay Diluent #9.

    • Linearity
      The concentrations of IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows:  mouse heart cells culture for 5 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1.17 ng/mL in mouse heart cells culture supernatant.

    • #2 sample is undiluted samples are as follows:  RAW264.7 cells stimulated with 1ug/ml LPS for 48h (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2.52 ng/mL in stimulated human RAW264.7 cells supernatant.

    • Leading Competitor comparison

    • Protocol Diagram

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