产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
IFN-α免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Precision
Intra-assay: 5.2%；
Inter-assay: 7.9%
Sample type
Cell culture supernatant, Serum, PlasmAssay type
Sandwich (quantitative)Sensitivity
1.097pg/mlRange
2.34 pg/mL - 150 pg/mLRecovery
Cell culture supernatant 89%
Serum 99%
Plasm 98%Assay time
60 minutesSpecies reactivity
HumanCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
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IFN-α is a cytokine that has an immunomodulatory function. It plays an important role not only in antiviral activity but also in several physiologic functions, such as activation of dendritic cells and accelerated expression of major histocompatibility complex I and II molecules that may cause increased antigen presentation. In humans, the type I IFN system consists of a family of IFN proteins encoded by at least 13 IFN alpha (IFNA) subtype genes (IFN-α1, -α2, -α4, -α5, -α6, -α7, -α8, -α10, -α13, -α14, -α16, -α17 and -α21), and one IFN beta gene (IFNB), one IFN-Epsilon gene, one IFN-Kappa gene, and IFN-Omega gene, all of which bind to the type I interferon receptor composed of the IFNAR1 and IFNAR2 chains.
ELISA
Standard curve
Example of Human IFN-α standard curve in Assay Diluent #1.Linearity
The concentrations of IFN-α were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 19.41 pg/mL in stimulated human PBMC supernatant.
Leading Competitor comparison
Protocol Diagram
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