产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
IL-15免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Precision
Intra-assay: 7.0%；
Inter-assay: 9.5%
Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
2.55pg/mlRange
15.63 pg/ml - 1000 pg/mlRecovery
Cell culture supernatant 95%
Serum 116%
Plasma 88%Assay time
60 minutesSpecies reactivity
HumanCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
The dose response curve of the International Standard (95/554) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (95/554) units, use the following equation: NIBSC (95/554) approximate value (IU/mL) = 0.01 x OneStep Human IL-15 value (pg/mL).
Interleukin-15 (IL-15) is a protein that in humans is encoded by the IL15 gene. IL-15 is an inflammatory cytokine with structural similarity to Interleukin-2 (IL-2). Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces the proliferation of natural killer cells, i.e. cells of the innate immune system whose principal role is to kill virally infected cells. IL-15 is constitutively expressed by a large number of cell types and tissues, including monocytes, macrophages, dendritic cells (DC), keratinocytes, fibroblasts, myocyte and nerve cells. As a pleiotropic cytokine, it plays an important role in innate and adaptive immunity.
ELISA
Standard curve
Example of Human IL-15 standard curve in Assay Diluent #4.Standard curve
Example of Human IL-15 standard curve in Assay Diluent #5.Leading Competitor comparison
Protocol Diagram
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