产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
IgG免疫原
Recombinant Protein抗体类型
Recombinant mAb纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
板内变异系数(Intra-assay): 4.0%；
板间变异系数(Inter-assay): 4.7%Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
102pg/mlRange
0.39 ng/ml - 25 ng/mlRecovery
Cell culture supernatant 86%
Serum 109%
Plasma 93%Assay time
60 minutesSpecies reactivity
RatCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
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There are four types of immunoglobulins in rats: IgA, IgE, IgM and IgG. Immunoglobulin G (IgG) is the most important form of immunoglobulin in serum, accounting for about 75% of the total content of immunoglobulins in serum, and it mainly participates in the recognition and elimination of pathogens and toxic substances, and has antiviral, antimicrobial agent, and immunomodulatory functions. IgG is a macromolecular glycoprotein with a molecular weight of 150 kDa, consisting of two light chains and two heavy chains, each of which is linked by a disulfide bond. is a macromolecular glycoprotein with a molecular weight of 150 kDa, consisting of two light chains and two heavy chains, each of which is connected to the other by a disulfide bond, forming a tetrameric quaternary structure. The resulting tetramer forms two identical hemispheres that together form a Y-like structure. The amino terminus, with its highly variable amino acid composition, is involved in antigen binding, whereas the constant portion of the C-terminus is involved in complement binding, placental passage, and cell membrane binding. In the rat, the IgG class is further divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG2c.
ELISA
Standard curve
Example of Rat IgG standard curve in Assay Diluent #1.Protocol Diagram
Linearity
The concentrations of rat IgG were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 Sample is undiluted samples are as follows: rat recombinant antibody supernatant (0.625%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2 ug/mL in rat recombinant antibody supernatant.
Linearity
#2 Sample is undiluted samples are as follows: rat serum(1:40000). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3.39 mg/mL in rat recombinant antibody supernatant.
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