产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
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抗原名称
GM-CSF免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
2 to 8 °C as supplied.
Precision
Intra-assay: 4.2%；
Inter-assay: 5.7%
Sample type
Cell culture supernatant, Serum, PlasmaAssay type
Sandwich (quantitative)Sensitivity
1.8 pg/mlRange
3.125 pg/ml - 200 pg/mlRecovery
Cell culture supernatant 88%
Serum 94%
Plasma 95%Assay time
60 minutesSpecies reactivity
HumanCROSS REACTIVITY
/INTERFERENCE
/NIBSC convert
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GM-CSF is a hematopoietic growth factor secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. GM-CSF binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and GM-CSF.
ELISA
Standard curve
Example of human GM-CSF standard curve in Assay Diluent #1.Linearity
The concentrations of GM-CSF were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 95 pg/mL in stimulated human PBMC supernatant.Leading Competitor comparison
Protocol Diagram
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