产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
大包装询价 产品介绍 引用文献(0) 评论(0)
抗原名称
IFN-γ免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
Precision
Intra-assay: 5.0%；
Inter-assay: 6.2%
Assay time
60 minutesSpecies reactivity
Human
Sample type
Cell culture supernatant, Serum, Plasma
Sensitivity
0.6 pg/ml
Range
3.4 pg/ml – 217.4 pg/ml
Recovery
Cell culture supernatant 105%Serum 83%
Plasma 84%Assay type
Sandwich (quantitative)NIBSC convert
The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFN-γ value (pg/mL).
Interferon gamma (IFN-γ) is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils. A non-IgE-mediated anaphylactic reaction and severe bronchospasm have been reported once after the first injection of interferon gamma. IFN-γ activates cells via a different receptor than IFN-α and IFN-β, which accounts for the different physiological properties of the proteins. Production of IFN-γ is largely restricted to activated CD4+ TH1 T cells, CD8+ T cells, and natural killer cells. One of the most important consequences of IFN-γ secretion is the activation of macrophages. In addition, IFN-γ plays a central role in inflammatory responses by activating endothelial cells, promoting TH1 cell development and cellular immune responses, and up-regulation of major histocompatability complex protein expression on antigen-presenting cells.
ELISA
Standard curve
Example of NIBSC (87/586) standard curve in Assay Diluent #1. Human IFN-γ standard were calibrated by NIBSC (87/586) with a calibration factor (CF) of 1.0. Human IFN-γ standard concentration = NIBSC (87/586)/CF
Standard curve
Example of human IFNγ standard curve in Assay Diluent #1.
Linearity
The concentrations of IFNγ were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 31,555 pg/mL in stimulated human PBMC supernatant.
#2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 112 pg/mL in unstimulated human PBMC supernatant
Leading Competitor comparison
Protocol Diagram
引用文献(0)
评论(0)