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抗体类型
Recombinant mAb应用
mIHC反应种属 ?
Hu标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用 | 稀释度 |
---|---|
mIHC | 1:200-1:1000 |
PD-L1, Cytotoxic T cell and Helper T cell (PD-L1, CD8α, CD4) Antibody Panel contains 3 recombinant monoclonal antibodies against PD-L1, CD8α, CD4. They are provided as a sampler panel.
Panel contains:
- S-RMab® PD-L1 Recombinant Rabbit mAb (SDT-R017) S0B2134
- CD8α Recombinant Mouse mAb (SDT-126-8) S0B2148
- CD4 Recombinant Rabbit mAb (SDT-R163) S0B2179
多重免疫组化
mIHC shows positive staining in paraffin-embedded human esophageal squamous cell carcinoma. Anti-CD8α antibody (S0B2148) was used at 1/1000 dilution (Green), Anti-PD-L1 antibody (S0B2134) was used at 1/200 dilution (magenta), Anti-CD4 antibody (S0B2179) was used at 1/500 dilution (white), followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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