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克隆号
S-3185抗体类型
Recombinant mAb纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
FCM
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
流式分析
Flow cytometric analysis of mouse splenocytes labeling Alexa Fluor® 488 Human IgG4 S228P, kappa isotype control at 1/20 dilution (1 μg) / (Right panel) compared with a unlable Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labeling Alexa Fluor® 488 Human IgG4 S228P, kappa isotype control at 1/20 dilution (1 μg) / (Right panel) compared with a unlable Control / (Left panel). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.







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