申请试用 p300 Recombinant Rabbit mAb (S-2612-122)
Histone acetyltransferase p300,p300 HAT,E1A-associated protein p300,Histone butyryltransferase p300,Histone crotonyltransferase p300,Protein 2-hydroxyisobutyryltransferase p300,Protein lactyltransferas p300,Protein propionyltransferase p300,EP300
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宿主来源
Rabbit抗原名称
p300分子别名
Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; Protein 2-hydroxyisobutyryltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300; EP300免疫原
Recombinant Protein细胞定位
Cytoplasm, NucleusAccession
Q09472克隆号
S-2612-122抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
293T, Jurkat, C2C12纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ? ,ICC ? ,WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Hu, Ms IHC-P 1:200 Hu ICC 1:500 Hu
p300 is a 2,414-residue human transcriptional coactivator that contains multiple conserved domains—bromodomain, CH1–CH3, HAT, KIX, and ZZ-type zinc finger—that enable it to serve as a histone acetyltransferase and a scaffold bridging sequence-specific DNA-binding factors with chromatin-remodeling machinery; by acetylating histones H3 and H4 as well as non-histone substrates such as p53 and NF-κB, it opens local chromatin structure and potentiates transcription, while its own activity is tightly regulated through phosphorylation, auto-acetylation, and competitive binding with viral oncoproteins like E1A or cellular partners like CBP; because it integrates proliferative, differentiation, and DNA-damage signals, p300 is indispensable during embryogenesis and is frequently altered—mutated, deleted, or translocated—in cancers, making it both a tumor suppressor and, in certain contexts, an oncogenic driver, and its HAT activity is pharmacologically targeted by small-molecule inhibitors under investigation for cancer therapy.
免疫印迹
WB result of p300 Recombinant Rabbit mAb
Primary antibody: p300 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 264 kDa
Observed MW: 280 kDa
The band below the target band is protein degradationWB result of p300 Recombinant Rabbit mAb
Primary antibody: p300 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 264 kDa
Observed MW: 280 kDa
The band below the target band is protein degradation
免疫组化
IHC shows positive staining in paraffin-embedded human testis. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-p300 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
免疫细胞化学
ICC shows positive staining in 293T cells. Anti- p300 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
组织表达图谱
Expression of p300 in tumor tissue.
Expression of p300 in human tissue.
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