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HDAC3 Recombinant Rabbit mAb (S-1977-31)

Histone deacetylase 3,HD3,Protein deacetylase HDAC3,Protein deacylase HDAC3,RPD3-2,SMAP45

价格 ￥600.00 （ 供应商现货 ： 3-5个工作日）
货号 S0B6614
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rabbit

  • 抗原名称

    HDAC3

  • 分子别名

    Histone deacetylase 3; HD3; Protein deacetylase HDAC3; Protein deacylase HDAC3; RPD3-2; SMAP45

  • 免疫原

    Synthetic Peptide

  • 细胞定位

    Cytoplasm, Nucleus

  • Accession

    O15379

  • 克隆号

    S-1977-31

  • 抗体类型

    Recombinant mAb

  • 抗体同种型

    IgG

  • 反应种属 ?

    Hu, Ms, Rt

  • 阳性样本

    HeLa, K562, Jurkat, HepG2, MCF7, NIN/3T3, mouse brain, PC-12, C6, rat brain

  • 预测反应种属
    (反应种属缩写表)

    Or

  • 纯化方式

    Protein A

  • 浓度

    0.5 mg/ml

  • 标记

    Unconjugated

  • 性状

    Liquid

  • 缓冲体系

    PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, -20 °C as supplied

  • 应用

    IHC-P ? ,
    WB

  • 稀释度

    应用 稀释度 推荐种属
    WB 1:1000 Hu, Ms, Rt
    IHC-P 1:500-1:2000 Hu, Ms, Rt
背景介绍

  • HDAC3 (histone deacetylase 3) is a 428-amino-acid zinc-dependent enzyme that removes acetyl groups from lysine residues on histones and many non-histone transcription factors, thereby compacting chromatin and repressing gene expression; it is an obligate component of the nuclear receptor corepressor (NCoR/SMRT) complex, shuttles between nucleus and cytoplasm through an intrinsic nuclear export signal, and its catalytic activity is allosterically activated by inositol tetraphosphate (IP4) bound within a conserved pocket, while global deletion in mice is embryonic-lethal and tissue-specific deletion reveals essential roles in circadian rhythm, immune tolerance, DNA repair, lipid and glucose metabolism, and neuronal function, making HDAC3 a major epigenetic drug target for cancer, metabolic and inflammatory diseases.

  • 免疫印迹

    • WB result of HDAC3 Recombinant Rabbit mAb
      Primary antibody: HDAC3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: HeLa whole cell lysate 20 µg
      Lane 2: K562 whole cell lysate 20 µg
      Lane 3: Jurkat whole cell lysate 20 µg
      Lane 4: HepG2 whole cell lysate 20 µg
      Lane 5: MCF7 whole cell lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 49 kDa
      Observed MW: 48 kDa

    • WB result of HDAC3 Recombinant Rabbit mAb
      Primary antibody: HDAC3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: NIH/3T3 whole cell lysate 20 µg
      Lane 2: mouse brain lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 49 kDa
      Observed MW: 48 kDa

    • WB result of HDAC3 Recombinant Rabbit mAb
      Primary antibody: HDAC3 Recombinant Rabbit mAb at 1/1000 dilution
      Lane 1: PC-12 whole cell lysate 20 µg
      Lane 2: C6 whole cell lysate 20 µg
      Lane 3: rat brain lysate 20 µg
      Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
      Predicted MW: 49 kDa
      Observed MW: 48 kDa

  • 免疫组化

    • IHC shows positive staining in paraffin-embedded human colon. Anti-HDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-HDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-HDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-HDAC3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded mouse kidney. Anti-HDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

    • IHC shows positive staining in paraffin-embedded rat testis. Anti-HDAC3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 组织表达图谱

    • Expression of HDAC3 in tumor tissue.

    • Expression of HDAC3 in human tissue.

    • Expression of HDAC3 in mouse & rat tissue.

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