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Rabbit抗原名称
CGRP分子别名
Calcitonin gene-related peptide 1; CGRP1; Alpha-type CGRP; Calcitonin gene-related peptide I (CGRP-I); CALC1; CALCA免疫原
Synthetic Peptide细胞定位
SecretedAccession
P06881克隆号
S-2490-74抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms, Rt阳性样本
TT纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
IHC-P ?
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Hu IHC-P 1:500 Hu, Ms, Rt
CGRP (calcitonin gene-related peptide) is a 37-amino-acid neuropeptide produced by tissue-specific splicing of the CALCA gene in primary afferent sensory neurons and central neurons; it signals through a heterodimeric receptor composed of the G-protein-coupled receptor CALCRL and the accessory protein RAMP1, potently dilating cerebral and dermal vasculature, modulating nociceptive transmission, and mediating neuro-inflammatory responses, making it a key target for monoclonal antibodies and small-molecule antagonists in the prevention of migraine and the treatment of cluster headache.
免疫印迹
WB result of CGRP Recombinant Rabbit mAb
Primary antibody: CGRP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: TT whole cell lysate 20 µg
Negative control: HeLa whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 14 kDa
Observed MW: 10 kDa
免疫组化
IHC shows positive staining in paraffin-embedded human spinal cord. Anti-CGRP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spinal cord (partial view). Anti-CGRP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spinal cord. Anti-CGRP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spinal cord. Anti-CGRP antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
组织表达图谱
Expression of CGRP in tumor tissue.
Expression of CGRP in human tissue.
Expression of CGRP in mouse & rat tissue.
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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