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Rabbit抗原名称
p16INK4a分子别名
Cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A (CDK4I); p16-INK4a (p16-INK4); P16ink4a; Cdkn2a免疫原
Synthetic Peptide细胞定位
Cytoplasm, NucleusAccession
P51480克隆号
S-2573-6抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
p16INK4a is a 16 kDa cyclin-dependent kinase inhibitor encoded by the CDKN2A locus on chromosome 9p21 that specifically binds to and inactivates CDK4/6 complexes, thereby preventing phosphorylation of the retinoblastoma protein (pRb) and enforcing a G1 cell-cycle arrest that functions as a critical tumor-suppressive barrier against oncogenic stress; its expression rises sharply with aging and acts as a robust biomarker of cellular senescence across diverse tissues, while its frequent inactivation through deletion, mutation, or promoter hypermethylation is observed in numerous human cancers, underscoring its pivotal role in both aging-related pathophysiology and oncogenesis.
免疫印迹
WB result of p16INK4a Recombinant Rabbit mAb
Primary antibody: p16INK4a Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: A20 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 18 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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