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宿主来源
Rabbit抗原名称
Sirt3分子别名
NAD-dependent protein deacetylase sirtuin-3; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3 (mSIR2L3); Sir2l3免疫原
Synthetic Peptide细胞定位
Cytoplasm, MitochondrionAccession
Q8R104克隆号
S-2005-62抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
mouse liver, mouse heart纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB稀释度
应用 稀释度 推荐种属 WB 1:1000-1:10000 Ms
Sirt3 protein, a member of the sirtuin family, is primarily located in the mitochondria. It is an NAD-dependent deacetylase that plays a crucial role in regulating various cellular processes. Sirt3 can deacetylate numerous target proteins, thereby influencing metabolism, stress resistance and aging. For example, it helps to enhance the activity of certain metabolic enzymes, promoting the breakdown of fatty acids and the generation of energy. Additionally, Sirt3 is involved in the response to oxidative stress, protecting cells from damage caused by reactive oxygen species. Its expression and activity are often altered in various diseases, making it a potential therapeutic target for conditions like metabolic disorders and neurodegenerative diseases.
免疫印迹
WB result of Sirt3 Recombinant Rabbit mAb
Primary antibody: Sirt3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Negative control: mouse spleen lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 29 kDa
FAQs
我们一般不推荐客户回收利用抗体。 因为抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，我们对一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定
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