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Sirt3 Recombinant Rabbit mAb (S-2004-6)

NAD-dependent protein deacetylase sirtuin-3,mitochondrial,hSIRT3,NAD-dependent protein delactylase sirtuin-3,Regulatory protein SIR2 homolog 3,SIR2-like protein 3,SIR2L3,SIRT3

Datasheet COA

价格￥600.00 （供应商现货： 3-5个工作日）

货号 S0B6082

规格

25μl100μl1ml

数量

产品介绍

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产品规格

宿主来源

Rabbit
抗原名称

Sirt3
分子别名

NAD-dependent protein deacetylase sirtuin-3, mitochondrial; hSIRT3; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; SIR2L3; SIRT3
免疫原

Synthetic Peptide
细胞定位

Mitochondrion
Accession

Q9NTG7
克隆号

S-2004-6
抗体类型

Recombinant mAb
抗体同种型

IgG
反应种属 ?

Hu, Ms, Rt
阳性样本

EK-293, PANC-1, mouse liver, rat liver, HaCAT
纯化方式

Protein A
浓度

0.5 mg/ml
标记

Unconjugated
性状

Liquid
缓冲体系

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件

12 months from date of receipt / reconstitution, -20 °C as supplied
应用

ICC ? ,
WB
稀释度

应用稀释度推荐种属

WB 1:1000 Hu, Ms, Rt
ICC 1:100 Hu

应用	稀释度	推荐种属
WB	1:1000	Hu, Ms, Rt
ICC	1:100	Hu

背景介绍

Sirt3, a member of the sirtuin family of proteins, is an important enzyme that is primarily located in the mitochondria. It functions as a deacetylase, removing acetyl groups from various proteins, which helps regulate their activity and stability. Sirt3 plays a crucial role in cellular metabolism, stress resistance, and aging processes. By modulating the activity of key metabolic enzymes, it helps maintain mitochondrial function and energy homeostasis. Additionally, Sirt3 is involved in protecting cells against oxidative stress by influencing the expression and activity of antioxidant enzymes. Its dysregulation has been implicated in various diseases, including metabolic disorders and cancer, making it a potential target for therapeutic interventions.

免疫印迹
- WB result of Sirt3 Recombinant Rabbit mAb
  Primary antibody: Sirt3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: HEK-293 whole cell lysate 20 µg
  Lane 2: PANC-1 whole cell lysate 20 µg
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 26 kDa
- WB result of Sirt3 Recombinant Rabbit mAb
  Primary antibody: Sirt3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: mouse spleen lysate 20 µg
  Lane 2: mouse liver lysate 20 µg
  Negative control: mouse spleen lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 26 kDa
- WB result of Sirt3 Recombinant Rabbit mAb
  Primary antibody: Sirt3 Recombinant Rabbit mAb at 1/1000 dilution
  Lane 1: rat spleen lysate 20 µg
  Lane 2: rat liver lysate 20 µg
  Negative control: rat spleen lysate
  Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
  Predicted MW: 43 kDa
  Observed MW: 26 kDa
免疫细胞化学
- ICC shows positive staining in HaCAT cells. Anti-Sirt3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).