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宿主来源
Rabbit抗原名称
FMC63 scFv免疫原
Recombinant Protein克隆号
S-2163-77抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A浓度
0.2 mg/ml标记
PE性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM稀释度
应用 稀释度 推荐种属 FCM 1μl per million cells in 100μl volume Species independent
FMC63 scFv (single-chain variable fragment) is a recombinant protein derived from the variable regions (VH and VL) of the anti-CD19 monoclonal antibody FMC63, linked by a flexible peptide spacer (e.g., (Gly₄Ser)₃). It specifically binds to the B-cell surface antigen CD19 with high affinity (KD ~1-10 nM) and is a key targeting component in FDA-approved CAR-T therapies, such as Kymriah®. The humanized FMC63 scFv retains potent antigen recognition while minimizing immunogenicity, making it highly effective in treating B-cell malignancies, including acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Ongoing research focuses on optimizing its affinity, stability, and functionality to enhance the safety and efficacy of CAR-T cell therapies.
流式分析
Flow cytometric analysis of FMC63 expression on CAR (FMC63 scFv) transfected 293T (Human embryonic kidney epithelial cell. CAR (FMC63 scFv) transfected 293T were stained with PE Rabbit IgG Isotype Control (Left panel) or SDT PE Rabbit Anti-FMC63 Antibody (Right panel) at 1 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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